Development and Validation of Uv Spectrophotometric Method for Determination of Hydroxychloroquine in Bulk and Tablet Formulation

Two simple, precise and economical UV spectrophotometric methods have been developed for the estimation of Hydroxychloroquine in pharmaceutical dosage form. Method A applied was area under curve (AUC) in which area under curve was integrated in the wavelength range of 251-261nm using water as a solvent. Method B involves getting first order derivative spectrum of drug solution and measurement of derivative amplitude at 347.80 nm. Calibration curves were plotted for both methods by using instrumental response at selected wavelength and concentrations of analyte in the solution. Linearity for the detector response was observed in the concentration range of 212 μg/ml for both the methods. The correlation coefficient for first and second method was 0.9981 and 0.9975 respectively. %Recovery from pharmaceutical formulation was 99.38-100.9 and 99.75-100.33 for first and second method respectively. The proposed methods were validated as per ICH analytical method development guidelines. Limit of detection and limit of quantitation were determined for both methods.